Asamizu Rei Target

DNA gel mobility shift assay of the promoter segments of putative target genes with His-LexA. DIG-labeled promoter segments of pilA7, pilA9, ggpS, slr1670, and psaD were incubated for 25 min at room temperature with His-LexA added at indicated concentrations. five-fold and 50-fold excess amounts of the non-labeled promoter segments were added as a competitor. Samples were separated on a 6% polyacrylamide gel.

This shows that gene transfers from the nucleus of the secondary endosymbiont to the nucleus of the secondary host cell were accompanied by acquisition of targeting pre-sequences that are suitable to re-target the gene product to its original location. This process is not trivial, since plastid targeting pre-sequences of red algae show completely different features than plastid targeting pre-sequences of diatoms [34], and pre-sequence acquisition is considered to be a crucial step in the evolutionary reduction of organellar genomes [35].

Asamizu Rei target

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